qPCR analysis for HDAC2 expression in SK-N-MC cells infected with HIV and/or treated with nicotine/vorinostat combination. SK-N-MC cells were infected with HIV and/or treated with nicotine. On the 5th day, 1 μg of vorinostat was added to the nicotine treated, HIV infected, HIV + nicotine treated cells. After total 7 days of HIV infection and/or treatment with nicotine, cells were harvested; RNA was isolated from the cells using illustra triplePrep Kit. The qPCR analysis indicated significantly increased HDAC2 expression in nicotine treated; HIV infected and in combined treatment. Vorinostat could able to inhibit the HDAC2 up-regulation in all the vorinostat treated test groups. (*, p ≤ 0.05; NS-Not Significant).