Western blot analysis for HDAC2 expression in SK-N-MC cells infected with HIV and/or treated with nicotine/vorinostat combination. SK-N-MC cells were infected with HIV and/or treated with nicotine. On the 5th day, 1 μg of vorinostat was added to the nicotine treated, HIV infected, HIV + nicotine treated cells. After total 7 days of HIV infection and/or treatment with nicotine, cells were harvested; protein was isolated from the cells using illustra triplePrep Kit. Transfer blots were probed with anti-HDAC2 antibody followed by secondary HRP conjugated antibody. Western blot results support the qPCR results indicating that HDAC2 up-regulation can be inhibited by vorinostat (at 1 μg conc.) in HIV and/or nicotine treated neuronal cells. (*, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; NS-Not Significant).