Figure 5

The conversion between full spikes and spikelets during AISS expression is influenced by the membrane potentials. A) After AISS onset under voltage-clamp, membrane potentials are hyperpolarized in the steps from −65 mV to −95 mV. A trace shows the spikes evoked just before AISS onset (black trace in the first part) and the AISS under different holding potentials at −65 (black), −75 (red), −85 (blue) and −95 mV (green) in a GABAergic neuron. Full spikes are converted into spikelets at holding potentials of −85 ~ −95 mV. B) shows the peak amplitudes of AISS under different membrane potentials of −65 (black), −75 (red), −85 (blue) and −95 mV (green). C) A trace illustrates the spikes evoked just before AISS onset (black trace in the first part) and the AISS under different holding potentials at −65 (black), −50 (red), −85 (blue) and −95 mV (green) in a GABAergic neuron. The spikelets are converted into full spikes at holding potential of −50 mV (red trace). D) shows the peak amplitudes of AISS under different holding potentials at −65 (black), −50 (red), −85 (blue) and −95 mV (green). E) shows the influence of membrane potential on AISS’s phase two, but not phase one. A trace under voltage-clamp recording shows the spikes evoked just before AISS expression (black trace) and the AISS at different holding potentials, −65 (black), −75 (red), −85 (blue) and −95 mV (green) in a GABAergic neuron. F) shows the values of slopes for phase one (open bars) and phase two (filled bars) under different holding potentials at −65 (black bar), −75 (red), −85 (blue) and −95 mV (green).