AISS induction is not affected by membrane potential hyperpolarization. AISS is induced under the voltage-clamp with the different holding potentials from −65 mV to −95 mV (n = 9). A) illustrates AISS induction under different membrane holding potentials at −65 (top trace), −75 (the second trace), −85 (the third trace) and −95 mV (bottom trace) from a GABAergic neuron. Calibration bars are 200 pA and 10 seconds. B) shows the number of spikes needed for inducing AISS under the different membrane holding potentials of −65, −75, −85 and −95 mV, in which the values for −75, −85 and −95 mV are normalized by the value for −65 mV. C) shows the frequencies of AISS induced under membrane holding potentials at −65, −75, −85 and −95 mV, in which the values for −75, −85 and −95 mV are normalized by the value for −65 mV.