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Figure 2 | Molecular Brain

Figure 2

From: The use of induced pluripotent stem cells to reveal pathogenic gene mutations and explore treatments for retinitis pigmentosa

Figure 2

The rhodopsin gene-targeting methods. (A, B) Schematic illustrations of the rhodopsin gene correction implemented in the RP#5 iPSCs and the mutagenesis in the 201B7 iPSCs using HDAdVs. (C) A PCR analysis confirmed that recombination occurred at the rhodopsin locus. Products of 4.2 kb and 15.8 kb were obtained using primers a-b and c-d (arrowheads in A, B), respectively. (D) Sequence analysis of the recombinant rhodopsin genes in each iPSC line. HDAdV, helper-dependent adenoviral vector; HSV-tk, herpes simplex virus thymidine kinase gene cassette; Neo, neomycin-resistance gene cassette; white triangles, loxP sites; red boxes, exon 3 of rhodopsin containing the E181K mutation. #5, RP#5 iPSCs; #5Rw, rhodopsin gene-corrected RP#5 iPSCs; B7, 201B7 iPSCs; B7Rm, rhodopsin gene-mutated 201B7 iPSCs.

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Molecular Brain

ISSN: 1756-6606