Figure 2

Expression of mHTT in monkey frontal cortex. A) Brain sections of HD monkey (rHD4, rHD7 and rHD8) and GFP monkey (C1 and C3) were immunostained with mEM48. Low magnification (upper panels, 40×) and high magnification (lower panels, 100×) shows that mHTT forms intranuclear inclusions (arrows) and neuropil aggregates (arrowheads). B) mEM48 immunoblot of frontal cortex showed high-molecular-mass mutant HTT aggregate (arrow) in the stacking gel. γ- tubulin was used as an internal control. C) Quantification of the mHTT aggregate in the stacking gel showed the relative level of mHTT aggregates in the frontal cortex. All HD monkeys have significantly higher level of mHTT aggregate compared to the controls. D) The percentage of mEM48 positive cells with or without intranuclear inclusions was calculated and compared among HD monkey frontal cortex immunostained with mEM48. HD7 has significantly more nuclei with intranuclear inclusions than HD4 and HD8. The data was presented as mean ± SE. *P < 0.05.