Figure 7

Effects of NB001 and gabapentin on zymosan-induced anxiety-like behaviors. (A) In the elevated plus maze, zymosan-treated mice administered gabapentin exhibited a significant reduction in anxiety-like behaviors on days 1, 7, 14 and 28 compared to mice administered saline (n = 6 mice per group for each testing day). * denotes a significant difference compared to saline- injected mice). No statistically significant difference was observed between zymosan-treated mice administered NB001 or saline on testing days (P = 0.43). (B) No statistically significant difference was observed in the total number of crossings (open + closed) in the elevated plus maze among zymosan-treated mice administered saline, gabapentin or NB001 on testing days (P = 0.80). (C) In the light/dark box test, zymosan-treated mice administered gabapentin exhibited a significant reduction in anxiety-like behaviors on days 1, 7, 14 and 28 compared to saline-injected mice (n = 6 mice per group for each testing day). * denotes a significant difference compared to saline-injected mice). There was no significant difference between zymosan-treated mice administered NB001 (n = 6 mice) or saline on testing days (P = 0.97). (D) No statistically significant difference was observed in the time until animals leave the dark box in the light/dark box test among zymosan-treated mice administered either saline, gabapentin or NB001 on testing days (P = 0.55). In each experiment, behaviors were measured 45 min after IP injection.