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Figure 3 | Molecular Brain

Figure 3

From: Postnatal maintenance of the 5-Ht1a-Pet1 autoregulatory loop by serotonin in the raphe nuclei of the brainstem

Figure 3

5-HT1A expression was decreased in the dorsal raphe nuclei of Tph2-/-mice. (A) qRT-PCR analysis of 5Ht1a, 5Ht1b and 5Ht1d in Tph2+/+ and Tph2-/- hindbrain. Tph2 mRNA level in the Tph2-/- hindbrain was used as a negative control. Relative expression was quantified using 18S rRNA as reference gene. (B) Immunostaining of 5-HT1A in the dorsal raphe nucleus, cortex, polymorph layer dentate gyrus (PoDG), inferior colliculus central nucleus (ICc) and superior colliculus (SC) of Tph2+/+ mice and Tph2-/- mice. (C) Quantification of 5-HT1A+ neurons in B7-8 raphe nucleus. Error bars represent SEM. n = 6. *P < 0.05, versus Tph2+/+, unpaired t test. Scale bars, 100 μm.

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