Skip to main content
Figure 4 | Molecular Brain

Figure 4

From: Loss of GABAergic cortical neurons underlies the neuropathology of Lafora disease

Figure 4

Increase of lysosomal activity in the cerebral cortex of EPM2A- /- mice. A) Confocal images of LAMP2 (green) and quantification of LAMP2 intensity in cerebral cortex of EPM2A-/-mice at 1 and 9 months of age (n = 5). Results are mean ± SEM ** p < 0.01 and *** p < 0.001 wild-type mice vs EPM2A -/- mice (ANOVA followed by Student’s t test). This quantification showed the increase of lysosomal activity in the cerebral cortex at late ages. B) Quantification of LAMP2 protein in cerebral cortex lsyates of EPM2A-/- mice (1 month-old, n = 5). Western blots represent the increase of the LAMP2 protein in the cerebral cortex lysates. C) F-actin accumulation in cerebral cortex of EPM2A-/- mice. Sections of cerebral cortex were stained with rhodamine-phalloidin. F-actin was detected in small clusters in the cell body of neurons in the cerebral cortex only in older laforin-deficient mice. The clusters of disarrangement of F-actin are pointed with white arrows in the representative picture.

Back to article page