Figure 5
From: Loss of GABAergic cortical neurons underlies the neuropathology of Lafora disease
Increase of caspase-3 activity in cortical neurons of EPM2A- /- mice. A) Confocal images of cerebral cortex sections of EPM2A-/- mice with active-caspase-3 (red) and GAD67 (green). B) Magnification of the microphotography in panel A. These representative images revealed the activation of caspase-3 in non-GABAergic neurons. The GABAergic neurons contacts mainly with glutamatergic neurons and these neurons seem to activate the caspase-3 in laforin-deficient mice. C) Representative western blot of active-caspase-3 and active-caspase-9 quantification in the lysates of cerebral cortex in EPM2A-/- mice (1Â month-old). D) Confocal images of cerebral cortex sections of EPM2A-/- mice with active-caspase-3 (green) and glutamatergic neuronal marker Glutamine synthetase Clone Gs-6 (red).