Figure 1
From: The development of suckling behavior of neonatal mice is regulated by birth
The original test procedure for measuring nipple attachment latency. (A) The experimental platform to test nipple attachment latency. The lactating dam was anesthetized and placed in a test cage. (B) The experimental procedure for measuring nipple attachment latency during development. Pups were separated from their mothers for 7 hours before the test. (C) The percentages of pups that were able to attach to a nipple within 180 sec. The percentage gradually increased after P4. (D) Latency between being placed in the cage and nipple attachment. Nipple attachment latency significantly decreased after P6 (*p < 0.05, Scheffe’s F test following Kruskal-Wallis test, mean ± SD).