Figure 2

Mutation of caveolin binding site of mGlu1 receptor induces its lipid rafts localization. Hippocampal primary neurons were transfected with SEP-mGlu1α receptor^wt (wild-type) and SEP-mGlu1α receptor^mu (mutant) constructs. After staining with CTX-Alexa 488, images were acquired with super-resolution microscopy, N-SIM. (A) White and orange boxes in upper images for soma and primary dendrites, respectively, are enlarged at the bottom. Overlapping areas (yellow) were indicated with asterisks, implying co-localization of mGlu1α receptor to CTX-Alexa 488. Upper and lower scale bars represent 5 μm and 1 μm. Co-localization values are obtained by Pearson’s correlation method. Values represent Pearson’s co-localization coefficient ± SEM. n = 11 (mGlu1α^wt) and 6 (mGlu1α^mu). Student’s t test, * P <0.05. (B) TIRF images visualizing co-localization of RFP-mGlu1α receptor wild-type (mGlu1α^wt) or RFP-mGlu1α receptor mutant (mGlu1α^mu) and lipid rafts (green). HEK293 cells were transfected with RFP-mGlu1α^wt or - mGlu1α^mu and labeled with CTX-Alexa 488 (green). Overlaying areas (yellow) indicate co-localization of mGlu1 receptorα with CTX-Alexa 488. n = 18 (mGlu1α^wt) and 18 (mGlu1α^mu). Scale bars = 10 μm. Co-localization scores are presented as average values ± SEM. Student’s t test, **, P < 0.01.