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Figure 2 | Molecular Brain

Figure 2

From: Gene delivery in mouse auditory brainstem and hindbrain using in utero electroporation

Figure 2

Schematic illustration depicting in utero injection and electroporation of Krox20 B enhancer-GFP construct into the 3rd ventricle of E12-13.5 embryonic mouse brain. Pregnant CD1 mouse (E12 to E13.5) was injected with CB-Krox20-GFP DNA construct or pCDNA3.1(−) (~1 to 2 μg) was injected to the lateral ventricle of mouse brain using glass capillary tip (Drummond Scientific, Broomall, PA, USA) directed towards the third ventricle to target the hindbrain. After DNA injection and electroporation embryos were carefully replaced back into the abdomen and the wound was closed. Detailed protocol is described in Methods.

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