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Figure 3 | Molecular Brain

Figure 3

From: Depletion of 14-3-3γ reduces the surface expression of Transient Receptor Potential Melastatin 4b (TRPM4b) Channels and attenuates TRPM4b-mediated glutamate-induced neuronal cell death

Figure 3

The current density of TRPM4b channel is increased by 14-3-3γ co-expression. (A) Surface biotinylation experiment was performed from HEK293T cells expressing GFP-TRPM4b with or without HA-14-3-3γ. Overexpression of 14-3-3γ markedly increased cell surface expression of TRPM4b. (B) Summary bar graph of TRPM4b level with or without 14-3-3γ co-expression was shown as a ratio of surface to total TRPM4b (n = 4). (C) The current densities were obtained from whole-cell currents of non-transfected HEK293T cells (black; n = 4), cells transfected with GFP-TRPM4b (green; n = 5), cells transfected with S88A TRPM4b mutant (red; n = 10) and cells co-transfected with GFP-TRPM4b and 14-3-3γ (blue; n = 6). Currents were activated by 1 μM ionomycin and the voltage-ramp (−100 mV to +100 mV). The current densities were calculated by subtracting the amplitude of initial currents from 1 μM ionomycin-activated currents and divided by cell capacitance. (D) Summary bar graph of TRPM4b currents with or without 14-3-3γ co-expression was plotted at +100 mV (** p < 0.01 and ***p < 0.001). All values are mean ± SEM.

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Molecular Brain

ISSN: 1756-6606