Figure 3

Confocal micrographs of retinal sections double-stained for synaptophysin and parvalbumin, an amacrine cell marker. At 4 weeks after intraocular pressure elevation, immunoreactivity for synaptophysin increased (A). Little co-staining with parvalbumin was seen (B). For cross-sectional immunohistochemical staining, n=6 for control and n=6 for glaucoma retinas at each time period; total n=24. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Scale bars=50 μm.