Figure 4

Confocal micrographs of flat-mounted retinas double-stained for synaptophysin and calretinin, an amacrine cell and retinal ganglion cell marker, was focused on the border between the ganglion cell layer and the inner plexiform layer. Celretinin stained the soma of the displaced amacrine cells and retinal ganglion cells in the ganglion cell layer. Immunoreactivity for synaptophysin increased at 1 (B), 4 (C), and 8 weeks (D) after intraocular pressure (IOP) elevation compared with the controls (A). Magnification at 4 weeks after IOP elevation (E). The area with both synaptophysin and calretinin co-staining did not change after IOP elevation (control set as 1.0, F). For flat mount immunohistochemical staining, n=6 for control and n=6 for glaucoma retinas at each time period; total n=24. Scale bars=50 μm.