Activation of the mTOR signalling pathway after acute LPS treatment. Representative immunoblots showed the immunoreactivity of mTOR, PS2448-mTOR, p70S6K and PT389-p70S6K in cortex (A and C) and in hippocampus (B and D) from treated-mice with a single () or two () or three () injections in i.p of LPS at 10mg/kg or control (0.9% NaCl). Mice were sacrificed 2, 4, 6 or 12 h after a single injection and 24 h after two and three injections. Semi-quantitative analysis of immunoblot was performed using Gene Tools software (Syngene, Ozyme France). The immunoreactivity of protein was normalized to β-tubulin immunoreactivity. The results are expressed as arbitrary units (% of 0.9% NaCl-injected mice group set at 100%). Results are mean±SEM for 6 mice in each group. *p < 0.01 compared to 0.9% NaCl-injected mouse group by Kruskal-Wallis test with a Dunns multiple comparison test.