Figure 6

Intrauterine injections reached the VM and do not alter the midbrain morphology. (A) Bright field and fluorescent images of an E14 brain injected in E12 with cell tracker and HA. (B) Hematoxylin and eosin staining of E14 ventral midbrain (VM) coronal sections of vehicle-injected or HA-injected embryos. (C) Comparison of E14 VM sagittal sections stained with hematoxylin and eosin from vehicle- and HA-injected embryos, where no histological differences were found. Scale bars: 500 μm and 150 μm. (D) To more quantitatively assess the VM, measurements of the thickness corresponding to the ventral part of the midbrain, where dopaminergic neurons are present, were performed; the brackets mark the regions used to measure thickness in both sides of the brain. The graph represents the average of thickness in both sides. No difference between vehicle- and HA-injected embryos was found. Scale bar: 150 μm.