Skip to main content
Figure 1 | Molecular Brain

Figure 1

From: TLR4 enhances histamine-mediated pruritus by potentiating TRPV1 activity

Figure 1

TLR4 enhances histamine-induced and chloroquine-induced itch sensation. A. Primary cultured DRG neurons were immunostained with NeuN and TLR4 antibodies. Scale bar, 50 μm. B. Primary sensory neurons from WT but not TLR4 KO mice were stained with Alexa488-conjugated LPS. Representative results are shown. Scale bar, 20 μm. C. LPS (100 or 500 μg) or histamine (100 μg) was introduced intradermally to WT mice under the nape. Scratching bouts were counted for 30 min (n = 5). LPS at either concentration did not induce significant scratching. D. WT or TLR4 KO mice were treated with pruritogens 5-HT 100 nmol, chloroquine (CQ) 200 μg, histamine 100 μg, imiquimod (R837) 200 μg, or SLIGRL 50 μg and scratching bouts were counted for 30 min (n = 6; **p < 0.01, *** p < 0.001). E. Histamine and chloroquine were introduced to WT and TLR4 KO mice at different doses and scratching bouts were measured (n = 5; **p < 0.01, ***p < 0.001). F. Histamine-receptor agonists HTMT (300 nmol) and clobenpropit (100 nmol), were used to treat WT and TLR4 KO mice and scratching bouts were counted (n = 6; ** p < 0.01).

Back to article page