Figure 4

Histamine receptor expression was not affected by TLR4. A. DRG neurons from WT and TLR4 KO mice were cultured and HRH1 was measured by western blot. A representative gel is shown (n = 3). B. DRG sections from WT and TLR4 KO mice were immunostained with HRH1 antibody. Representative data are shown (n = 3). Scale bar, 50 μm. HRH1-expressing neuronal percentage and size distribution were not noticeably altered in TLR4 KO DRG. C and D. The mRNA expression of histamine receptors (HRH1 and HRH4) (C) and MrgprA3 (D) were measured by real-time RT-PCR in WT and TLR4 KO sensory neurons (n = 3). The MrgprA3 expression is significantly reduced in TLR4 KO neurons (*p < 0.005).