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Figure 6 | Molecular Brain

Figure 6

From: TLR4 enhances histamine-mediated pruritus by potentiating TRPV1 activity

Figure 6

TLR4 expression enhances TRPV1 activity in HEK293T cells. A. HEK293T cells transiently overexpressing TRPV1 or TRPV1 plus TLR4 were loaded with Fura2-AM. Cells were treated with capsaicin (10 μM) followed by ionomycin (0.3 μg/ml) and intracellular calcium level was measured by population assay. Representative traces are shown. B. Average net increase of [Ca++]i (Δ ratio (340 nm/380 nm)) is shown in a graph (n = 6). C. Whole cell patch clamp recording was performed using HEK293T cells transiently overexpressing TRPV1 or TRPV1 plus TLR4. Representative traces of capsaicin-induce inward currents are shown. D. The average amplitude of the capsaicin-induced inward current in TRPV1-alone or TRPV1-plus-TLR4-expressing HEK293T cells (n = 18; *p < 0.005). The inward current in TLR4-expressing cells is six times the current of TLR4-deficient cells.

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