Figure 5

The regulation of spine dynamics by STEP. (A) Representative images of the same dendrite at two time points from wild type, STEP KO and STEP KO plus L689,560. Scale bar (applies to all panels): 5 μm. (B-D). Analysis of ER- spines. (B) Cumulative probability plot of ER- spine volume at t = 0 for wild type, STEP KO and STEP KO pre-treated for 10 min with L689,560 (K-S test, P < 0.001 between WT and STEP KO and P < 0.001 between STEP KO and STEP KO with L689,560). (C) Equivalent plots for normalised spine volumes. (D) Spine distributions for the three groups (Z test, P < 0.05 between STEP KO and STEP KO with L689,560 for stable and shrinking). (E-G) Equivalent plots for ER + spines. (E) Cumulative probability plot of ER + spine volume at t = 0 for wild type, STEP KO and STEP KO with L689,560 (K-S test, P < 0.001 between WT and STEP KO and P < 0.001 between STEP KO and STEP KO with L689,560). (F) Equivalent plots for normalised spine volumes (K-S test, P < 0.01 between WT and STEP KO and P < 0.001 between STEP KO and STEP KO with L689,560). (G) Spine distributions for the three groups (Z test, WT versus STEP KO or STEP KO versus STEP KO with L689,560).