Intra-mPFC infusion of muscimol impairs the performance of rats on the delayed alternation task. (A) Infusion sites of muscimol in the mPFC, as indicated by the arrow. (B) The correct rate of task performance was reduced upon muscimol infusion. (C) The win-shift and lose-shift strategies were destroyed after muscimol treatment, with a significant increase in win-shift and lose-shift failures. (D) Reaction time (RT) was unchanged upon muscimol infusion. RT was defined as the duration from the delay off to the breaking of infrared beam #4 or #6. ***p < 0.001, n = 9 rats, paired t-test. Cg1, cingulate cortex, area 1; IL, infralimbic cortex; PrL, prelimbic cortex.