Generation of a line of transgenic mice that overexpress SIRT1 (PrP-Sirt1). (A) Schematic diagram of transgenes used to generate PrP-Sirt1 founder mice. Full-length mouse Sirt1 cDNA was subcloned into the MoPrP (mouse prion promoter)-vector . The non-coding portion of exon 2 and 3, and the Sirt1 cDNA insert (with its own start and stop codon) were fused into one exon. The linearized transcription unit was co-injected with a visual marker, α-crystallin promoter-driven enhanced green fluorescent protein (EGFP) cDNA. (B) SIRT1 expression in PrP-Sirt1 and non-transgenic (nTg) mouse spinal cord. The expression level of Sirt1 was quantified in three independent experiments and shown as mean ± standard error of the mean (SEM). The data was analyzed by Student’s t-test. (C) Survival curve of the PrP-Sirt1 and nTg mice plotted over time (n = 28 each). Mean survival time (days) was shown with standard deviation (SD).