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Figure 2 | Molecular Brain

Figure 2

From: SIRT1 overexpression ameliorates a mouse model of SOD1-linked amyotrophic lateral sclerosis via HSF1/HSP70i chaperone system

Figure 2

Expression of endogenous SIRT1 and the major heat shock proteins in the spinal cord of SOD1G93A, SOD1G37Ror SIRT1 transgenic mice. (A) Endogenous SIRT1 and HSP70i were upregulated in high copy SOD1G93A mouse (SOD1G93A-H) spinal cord. A representative immunoblot image for SIRT1 and HSP70i in the lumbar spinal cord of SOD1G93A-H, SOD1G93A-H and PrP-Sirt1 double transgenic (SOD1G93A-H/PrP-Sirt1), or non-transgenic (nTg) mice at designated age. Each lane contained 30 μg total protein and the similar results were obtained from three independent experiments. (B) Endogenous SIRT1 was upregulated in SOD1G37R mouse spinal cord. A representative immunoblot image for SIRT1 in the lumbar spinal cord of SOD1G37R, PrP-Sirt1, or nTg mice at designated age. Each lane contained 20 μg total protein and the similar results were obtained from three independent experiments. (C) HSP70i was modestly induced in SOD1G37R mouse spinal cord. Representative immunoblot images for HSP70i, HSP90, and HSP110 in the same lumber spinal cord homogenates as in (B). Each lane contained 30 μg total protein and the similar results were obtained from three independent experiments.

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