Table 1 Summary of systematic behavioral tests for PrP-Sirt1 mice in comparison with wild-type littermates
Tests | Mental/physical activities | Indices measured | Alteration from wild type |
|---|---|---|---|
| Â | Â | (inexhaustive) | PrP-Sirt1 |
General health and neurological screening | General health | Body weight | ↓ |
(Additional file 1: Figure S3) |  | Rectal temperature | → |
|  |  | Grip strength | → |
|  |  | Hanging persistence | ↓ |
Light/dark transition test | Exploratory activity | Distance traveled in the light chamber | → |
(Additional file 1: Figure S4) | Light avoidance | Distance traveled in the dark chamber | → |
|  |  | Latency to the first entry to the light chamber | → |
|  |  | Time stayed in the light chamber | → |
|  |  | Number of transitions between chambers | ↓ |
Open field test | Exploratory activity | Distance traveled | ↑ |
(Additional file 1: Figure S5) | Avoidance from open space | Center time | → |
|  | Anxiety-like behavior | Vertical activity | → |
|  |  | Stereotypic counts | → |
Elevated plus maze test | Exploratory activity | Distance traveled | → |
(Additional file 1: Figure S6) | Height avoidance | Entries into open arms | → |
|  |  | Number of entries | → |
|  |  | Time stayed on open arms | → |
Acoustic startle response | Startle reflex to loudness | Ampliture of body motion | → |
(Additional file 1: Figure S7A) | Â | Â | Â |
Prepulse inhibition (PPI) test | Sensorimotor gating | Decrement of startle amplitude | → |
(Additional file 1: Figure S7B) | Â | Â | Â |
Porsolt forced swim test | Despair-like behavior | Latency to immobility | → |
(Additional file 1: Figure S8) | Â | Â | Â |
Home cage monitoring | Diurnal cycle of locomotor activity | Activity level (distance traveled) | → |
(Additional file 1: Figure S9) | Social behavior | Mean number of particles | → |
Social interaction test | Social behavior, anxiety-like behavior | Distance traveled | → |
(1 chamber, stranger pair) |  | Number of contacts | → |
(Additional file 1: Figure S10) |  | Total duration of active contacts | → |
|  |  | Mean contact duration | → |
|  |  | Total duration of contacts | → |
Social interaction test | Social behavior, anxiety-like behavior | Time spent with novel stranger | ↓ (Step 2) |
(3 chamber, 1–2 caged strangers) |  |  |  |
(Additional file 1: Figure S11) |  | Distance traveled | → |
Rota-rod test | Motor coordination/learning | Latency to fall | → |
(Additional file 1: Figure S12) | Â | Â | Â |
Gait analysis | Mechanics of limb movement | Limb positions and timings | → (front step angle) |
(Additional file 1: Figure S13) |  |  | ↑ (hind step angle) |
Hot plate test | Aversive response to noxious stimulus | Latency to limb withdrawal | → |
(Additional file 1: Figure S14) | Â | Â | Â |
Tail suspension test | Behavioral despair | Latency to immobility | → |
(Additional file 1: Figure S15) | Â | Â | Â |
Barnes maze | Spatial memory | Time spent around each hole | → |
(Additional file 1: Figure S16) | Â | Â | Â |
Fear conditioning test | Fear memory | Conditioning | → |
(Additional file 1: Figure S17) |  | Contextual testing | → |
|  |  | Cued test with altered context | → |