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Table 1 Summary of systematic behavioral tests for PrP-Sirt1 mice in comparison with wild-type littermates

From: SIRT1 overexpression ameliorates a mouse model of SOD1-linked amyotrophic lateral sclerosis via HSF1/HSP70i chaperone system

Tests Mental/physical activities Indices measured Alteration from wild type
   (inexhaustive) PrP-Sirt1
General health and neurological screening General health Body weight
(Additional file 1: Figure S3)   Rectal temperature
   Grip strength
   Hanging persistence
Light/dark transition test Exploratory activity Distance traveled in the light chamber
(Additional file 1: Figure S4) Light avoidance Distance traveled in the dark chamber
   Latency to the first entry to the light chamber
   Time stayed in the light chamber
   Number of transitions between chambers
Open field test Exploratory activity Distance traveled
(Additional file 1: Figure S5) Avoidance from open space Center time
  Anxiety-like behavior Vertical activity
   Stereotypic counts
Elevated plus maze test Exploratory activity Distance traveled
(Additional file 1: Figure S6) Height avoidance Entries into open arms
   Number of entries
   Time stayed on open arms
Acoustic startle response Startle reflex to loudness Ampliture of body motion
(Additional file 1: Figure S7A)    
Prepulse inhibition (PPI) test Sensorimotor gating Decrement of startle amplitude
(Additional file 1: Figure S7B)    
Porsolt forced swim test Despair-like behavior Latency to immobility
(Additional file 1: Figure S8)    
Home cage monitoring Diurnal cycle of locomotor activity Activity level (distance traveled)
(Additional file 1: Figure S9) Social behavior Mean number of particles
Social interaction test Social behavior, anxiety-like behavior Distance traveled
(1 chamber, stranger pair)   Number of contacts
(Additional file 1: Figure S10)   Total duration of active contacts
   Mean contact duration
   Total duration of contacts
Social interaction test Social behavior, anxiety-like behavior Time spent with novel stranger ↓ (Step 2)
(3 chamber, 1–2 caged strangers)    
(Additional file 1: Figure S11)   Distance traveled
Rota-rod test Motor coordination/learning Latency to fall
(Additional file 1: Figure S12)    
Gait analysis Mechanics of limb movement Limb positions and timings → (front step angle)
(Additional file 1: Figure S13)    ↑ (hind step angle)
Hot plate test Aversive response to noxious stimulus Latency to limb withdrawal
(Additional file 1: Figure S14)    
Tail suspension test Behavioral despair Latency to immobility
(Additional file 1: Figure S15)    
Barnes maze Spatial memory Time spent around each hole
(Additional file 1: Figure S16)    
Fear conditioning test Fear memory Conditioning
(Additional file 1: Figure S17)   Contextual testing
   Cued test with altered context
  1. Comparative table of behavioral test results with PrP-Sirt1 and wild-type littermate C57BL/6 J mice. Symbols: →, no significant difference; ↑↓, statistically significant increase and decrease as compared with wild-type control mice. N.D., not determined.