Figure 10

Transfection of human fetal neuron culture using RNAiMAX transfection agent and APP siRNA demonstrates transfectability of cells. Cells were transfected at DIV19 in a 24-well cell culture plate using 1.75 μL RNAiMAX (Invitrogen) and 20 nM APP siRNA (Applied Biosystems) as per manufacturer's instructions. To improve clarity, we have drawn boxes to separate groups. Cell lysates were prepared 72 h post-transfection and APP levels (22C11, Millipore; Cat #MAB348) assayed by Western blot. APP levels were reduced by over 50% following transfection of APP siRNA. *p < 0.05 by Student's t-test.