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Table 2 List of different primary and secondary antibodies used to characterize the culture

From: Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research

Primary antibody Dilutions Company Cat.no. Secondary antibody Dilutions Company Cat.no.
Panneuroral marker (ICC) 1:3000 Millipore MAB2300 Biotinylated Donkey anti mouse 1:500 Jackson Immuno Research 715-065-150
Glial Fibrillary Acidic protein (GFAP) (WB) 1:20,000 Sigma G9269 HRP-conj. Goat anti Rabbit 1:7,500 Thermo Scientific 31460
Glial Fibrillary Acidic protein (GFAP) (ICC) 1:10,000 Sigma G9269 Cy3-conj. Donkey anti Rabbit 1:500 Jackson Immuno Research 711-165-152
Nestin (ICC) 1:1000 Sigma N5413 Cy3-conj. Donkey anti Rabbit 1:500 Jackson Immuno Research 711-165-152
Neuron-specific Enolase (NSE) (WB) 1:3000 Abcam ab16873 HRP-conj. Goat anti Rabbit 1:7,500 Thermo Scientific 31460
Synaptosomal-associated Protein of 25 kDa (SNAP25) (WB) 1:5000 Millipore MAB331 HRP-conj. Goat anti Mouse 1:3000 Rockland 610-1319
Post Synaptic Density Protein of 95 kDa (PSD-95) (WB) 1:3000 NeuroMab 75-028 HRP-conj. Goat anti Mouse 1:3000 Rockland 610-1319
β actin (WB) 1:100,000 Sigma A5441 HRP-conj. Goat anti Mouse 1:3000 Rockland 610-1319
5-Hydroxytryptamine (5-HT) (ICC) 1:1000 ImmunoStar 20080 Cy3-conj. Donkey anti Rabbit 1:500 Jackson Immuno Research 711-165-152
Glulamate Decarboxylase (GAD65/67) (WB) 1:1000 Millipore AB1511 HRP-conj. Goat anti Rabbit 1:7,500 Thermo Scientific 31460
Tyrosine Hyroxylase (WB) 1:2000 Millipore MAB318 HRP-conj. Goat anti Mouse 1:3000 Rockland 610-1319
Amyloid Precursor Protein (APP) (22C11 clone) 1:3000 Millipore MAB348 HRP-conj. Goat anti Mouse 1:3000 Rockland 610-1319
α-tubulin 1:100,000 Sigma T9026 HRP-conj. Goat anti Mouse 1:3000 Rockland 610-1319