Primary antibody | Dilutions | Company | Cat.no. | Secondary antibody | Dilutions | Company | Cat.no. |
---|---|---|---|---|---|---|---|
Panneuroral marker (ICC) | 1:3000 | Millipore | MAB2300 | Biotinylated Donkey anti mouse | 1:500 | Jackson Immuno Research | 715-065-150 |
Glial Fibrillary Acidic protein (GFAP) (WB) | 1:20,000 | Sigma | G9269 | HRP-conj. Goat anti Rabbit | 1:7,500 | Thermo Scientific | 31460 |
Glial Fibrillary Acidic protein (GFAP) (ICC) | 1:10,000 | Sigma | G9269 | Cy3-conj. Donkey anti Rabbit | 1:500 | Jackson Immuno Research | 711-165-152 |
Nestin (ICC) | 1:1000 | Sigma | N5413 | Cy3-conj. Donkey anti Rabbit | 1:500 | Jackson Immuno Research | 711-165-152 |
Neuron-specific Enolase (NSE) (WB) | 1:3000 | Abcam | ab16873 | HRP-conj. Goat anti Rabbit | 1:7,500 | Thermo Scientific | 31460 |
Synaptosomal-associated Protein of 25 kDa (SNAP25) (WB) | 1:5000 | Millipore | MAB331 | HRP-conj. Goat anti Mouse | 1:3000 | Rockland | 610-1319 |
Post Synaptic Density Protein of 95 kDa (PSD-95) (WB) | 1:3000 | NeuroMab | 75-028 | HRP-conj. Goat anti Mouse | 1:3000 | Rockland | 610-1319 |
β actin (WB) | 1:100,000 | Sigma | A5441 | HRP-conj. Goat anti Mouse | 1:3000 | Rockland | 610-1319 |
5-Hydroxytryptamine (5-HT) (ICC) | 1:1000 | ImmunoStar | 20080 | Cy3-conj. Donkey anti Rabbit | 1:500 | Jackson Immuno Research | 711-165-152 |
Glulamate Decarboxylase (GAD65/67) (WB) | 1:1000 | Millipore | AB1511 | HRP-conj. Goat anti Rabbit | 1:7,500 | Thermo Scientific | 31460 |
Tyrosine Hyroxylase (WB) | 1:2000 | Millipore | MAB318 | HRP-conj. Goat anti Mouse | 1:3000 | Rockland | 610-1319 |
Amyloid Precursor Protein (APP) (22C11 clone) | 1:3000 | Millipore | MAB348 | HRP-conj. Goat anti Mouse | 1:3000 | Rockland | 610-1319 |
α-tubulin | 1:100,000 | Sigma | T9026 | HRP-conj. Goat anti Mouse | 1:3000 | Rockland | 610-1319 |