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Table 2 List of different primary and secondary antibodies used to characterize the culture

From: Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research

Primary antibody

Dilutions

Company

Cat.no.

Secondary antibody

Dilutions

Company

Cat.no.

Panneuroral marker (ICC)

1:3000

Millipore

MAB2300

Biotinylated Donkey anti mouse

1:500

Jackson Immuno Research

715-065-150

Glial Fibrillary Acidic protein (GFAP) (WB)

1:20,000

Sigma

G9269

HRP-conj. Goat anti Rabbit

1:7,500

Thermo Scientific

31460

Glial Fibrillary Acidic protein (GFAP) (ICC)

1:10,000

Sigma

G9269

Cy3-conj. Donkey anti Rabbit

1:500

Jackson Immuno Research

711-165-152

Nestin (ICC)

1:1000

Sigma

N5413

Cy3-conj. Donkey anti Rabbit

1:500

Jackson Immuno Research

711-165-152

Neuron-specific Enolase (NSE) (WB)

1:3000

Abcam

ab16873

HRP-conj. Goat anti Rabbit

1:7,500

Thermo Scientific

31460

Synaptosomal-associated Protein of 25 kDa (SNAP25) (WB)

1:5000

Millipore

MAB331

HRP-conj. Goat anti Mouse

1:3000

Rockland

610-1319

Post Synaptic Density Protein of 95 kDa (PSD-95) (WB)

1:3000

NeuroMab

75-028

HRP-conj. Goat anti Mouse

1:3000

Rockland

610-1319

β actin (WB)

1:100,000

Sigma

A5441

HRP-conj. Goat anti Mouse

1:3000

Rockland

610-1319

5-Hydroxytryptamine (5-HT) (ICC)

1:1000

ImmunoStar

20080

Cy3-conj. Donkey anti Rabbit

1:500

Jackson Immuno Research

711-165-152

Glulamate Decarboxylase (GAD65/67) (WB)

1:1000

Millipore

AB1511

HRP-conj. Goat anti Rabbit

1:7,500

Thermo Scientific

31460

Tyrosine Hyroxylase (WB)

1:2000

Millipore

MAB318

HRP-conj. Goat anti Mouse

1:3000

Rockland

610-1319

Amyloid Precursor Protein (APP) (22C11 clone)

1:3000

Millipore

MAB348

HRP-conj. Goat anti Mouse

1:3000

Rockland

610-1319

α-tubulin

1:100,000

Sigma

T9026

HRP-conj. Goat anti Mouse

1:3000

Rockland

610-1319