Figure 5
Reductions by MgT treatment in amyloid plaques and BACE1 overexpression in APPswe/PS1dE9 transgenic mice (Tg mice). (A) Upper panel left: Immunostaining of hippocampal amyloid plaque of Tg (n = 6) and Tg + MgT (n = 5). Right: Hippocampal amyloid plaque areas were significantly lower in Tg + MgT mice. Lower panel: same as above but in the frontal cortex. Two tailed unpaired t-test, * p < 0.05. (B) Concentrations of Aβ42 (upper panel) and Aβ40 (lower panel) monomers in CSF of Tg (n = 13) and Tg + MgT (n = 15) measured by ELISA. (C) Left: Representative Western blots of BACE1 (β-secretase) expression in the hippocampus of mice sacrificed under home-cage conditions (basal) or following 24 h of environmental enrichment-based stimulation (Stim). Middle: Quantitative analysis of BACE1 expression in the hippocampus of WT, Tg and Tg + MgT mice (n = 5/group) obtained by Western blot (arbitrarily units, a.u.). Mice were sacrificed under basal conditions without behavioral stimulation. Right: Quantitative analysis of BACE1 expression in the hippocampus of WT (n = 6), Tg (n = 6) and Tg + MgT mice (n = 5) obtained by Western blots following behavioral stimulation. ANOVAs (Table2) were followed by Bonferroni's post hoc test. (D) Left: Representative Western blots of hsAPPβ expression in the hippocampus of mice sacrificed under home-cage conditions (basal) or following 24 h of environmental enrichment-based stimulation (Stim). Middle: Quantitative analysis of hsAPPβ expression in the hippocampus of Tg and Tg + MgT mice (n = 5/group) obtained by Western blot (arbitrarily units, a.u.). Mice were sacrificed under basal conditions without behavioral stimulation. Right: Quantitative analysis of hsAPPβ expression in the hippocampus of Tg (n = 6) and Tg + MgT mice (n = 5) obtained by Western blots following behavioral stimulation. (E) Same as (D) but for β-CTF, Tg (n = 3) obtained by Western blots following behavioral stimulation. Two tailed unpaired t-tests. Data from WT mice was displayed but not included in the analysis. Error bars show SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.