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Table 3 Primary and secondary antibodies

From: Elevation of brain magnesium prevents synaptic loss and reverses cognitive deficits in Alzheimer’s disease mouse model

Experiments Primary antibodies Host Dilution Source
Quantitative immunostaining Synaptophysin Mouse 1:500 Millipore
GAD65 Rabbit 1:500 Millipore
Western blot NR2B Rabbit 1:6000 Santa Cruz
pCamkII (THr286) Rabbit 1:1000 Cell Signaling
CamkII Rabbit 1:1000 Cell Signaling
pCreb (Ser133) Rabbit 1:50 Cell Signaling
Creb Rabbit 1:1000 Cell Signaling
GAPDH Rabbit 1:6000 Cell Signaling
BACE1 Rabbit 1:500 Cell Signaling
6E10 (β-CTF) mouse 1:6000 Signet
hsAPPβ Rabbit 1:50 IBL
Experiments Secondary antibodies Dilution Source
Quantitative immunostaining CF488 Conjugated Goat anti-Mouse IgG(H + L) 1:200 Biotium
CF488 Conjugated Goat anti-Rabbit IgG(H + L) 1:200 Biotium
Western blot HRP-conjugated Goat anti-Rabbit IgG(H + L) 1:20000 ZSGB-BIO
HRP-conjugated Goat anti-Mouse IgG(H + L) 1:25000 CST