Table 3 Primary and secondary antibodies
Experiments | Primary antibodies | Host | Dilution | Source |
|---|---|---|---|---|
Quantitative immunostaining | Synaptophysin | Mouse | 1:500 | Millipore |
GAD65 | Rabbit | 1:500 | Millipore | |
Western blot | NR2B | Rabbit | 1:6000 | Santa Cruz |
pCamkII (THr286) | Rabbit | 1:1000 | Cell Signaling | |
CamkII | Rabbit | 1:1000 | Cell Signaling | |
pCreb (Ser133) | Rabbit | 1:50 | Cell Signaling | |
Creb | Rabbit | 1:1000 | Cell Signaling | |
GAPDH | Rabbit | 1:6000 | Cell Signaling | |
BACE1 | Rabbit | 1:500 | Cell Signaling | |
6E10 (β-CTF) | mouse | 1:6000 | Signet | |
hsAPPβ | Rabbit | 1:50 | IBL | |
Experiments | Secondary antibodies | Dilution | Source | |
Quantitative immunostaining | CF488 Conjugated Goat anti-Mouse IgG(H + L) | 1:200 | Biotium | |
CF488 Conjugated Goat anti-Rabbit IgG(H + L) | 1:200 | Biotium | ||
Western blot | HRP-conjugated Goat anti-Rabbit IgG(H + L) | 1:20000 | ZSGB-BIO | |
HRP-conjugated Goat anti-Mouse IgG(H + L) | 1:25000 | CST | ||