Figure 6

Time course of c-Fos expression. The numbers of c-Fos-positive cells in the four brain regions (mPOA (A), VTA (B), NAcc (C) and VT (D), respectively) of the sires were counted from photomicrographs of coronal sections of c-Fos immunoreactivity with a time window of 30, 60 and 120 min from the start of separation in new cages together with the mate dam. The sire just before isolation (the S1 condition) was used for time zero. Sires in the nonretriever (blue bars, S4-1) and retriever (brown bars, S4-2) families. Statistical analysis of two-way ANOVA is listed in Table 2. One-way ANOVA followed by Bonferroni's post-hoc test revealed significant differences between non-stimulation and each time point at ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 from S1; ^dP < 0.001 from 30 min; ^eP < 0.001 from 120 min, respectively.