Figure 4
From: 5-mehtyltetrahydrofolate rescues alcohol-induced neural crest cell migration abnormalities
5-mehtyltetrahydrofolate improves alcohol-induced developmental abnormalities. (A) Homocysteine was significantly increased at stage 27 (from 25.5±3.7 micromole/L /100embryos in control group to 41.8±6.3 micromole/L /100embryos in alcohol group, P < 0.05), whereas the alteration is undetectable statistically at stage 16(23.5±2.5 micromole/L /100embryos in control and 23.0±2.3 micromole/L /100embryos in alcohol group, P > 0.05). Supplementation of 5MTHF significantly (P < 0.05) buffers the accumulation of endogenous homocysteine to 30.5±2.3 micromole/L /100embryos at stage 27. (B,C) Injection of 5MTHF restored neural crest migration, and alleviated the effect of alcohol on neural crest migration. (D) Schematic diagram illustrating the xenografting experiments. (E-G) Alcohol exposure partially or completely blocked GFP-labeled neural crest xenografting migrating in living embryos. (H) Quantification of the GFP-labeled Neural crest migation upon gradient alcohol exposure and 5MTHF treatment.