Figure 2From: Multiple microRNAs regulate human FOXP2 gene expression by targeting sequences in its 3' untranslated regionMultiple miRNAs downregulate human FOXP2 expression. (A) Representative Western blot images and quantitative results showing that miR-9, miR-19b, miR-140-5p, miR-200a, let-7a, miR-129-5p, miR-582-5p, and miR-892a downregulated human FOXP2 protein expression when transfected into HEK293 cells. (B and C) The downregulatory effects of let-7a, miR-9, and miR-129-5p were dosage-dependent. (D) Let-7a, miR-9, and miR-129-5p downregulated FOXP2 mRNA expression in similar transfection experiments as measured by qRT-PCR. For both Western blot and qRT-PCR, Actin was used as an internal control; a miRNA mimic with a scrambled sequence (Ambion) was used as a negative control; and a siRNA against FOXP2 siFOXP2 was used as a positive control. Transfection was performed at least three times (n'='3) for each miRNA, and quantifications were averaged from the three transfections, two Western blot or two qRT-PCR experiments per transfection. *p<0.05, **p<0.01, and ***p<0.001.Back to article page