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Figure 8 | Molecular Brain

Figure 8

From: Sensitization of neurons in the central nucleus of the amygdala via the decreased GABAergic inhibition contributes to the development of neuropathic pain-related anxiety-like behaviors in rats

Figure 8

Alteration of the proportion of GABAergic neurons and expression of GAD65, GAD67, VGLUT1 and VGLUT2 in the CeA in SNL-induced anxiety rats. (A): proportion of GABAergic neurons. Note that the proportion of GABAergic neurons in the CeA is decreased significantly in SNL rats compared to na''ve and sham rats (n = 25 cells from 5 na''ve rats, 15 cells from 4 sham rats and 25 cells from 5 SNL rats). (B - C): Western blot detection of GAD65 and GAD67 expression in CeA nucleus. Upper: representative of Western blot bands; Lower: analysis of the relative intensity of GAD65 and GAD67. β-actin is used as an internal control. Note that the expression of both GAD65 (B) and GAD67 (C) are statistically decreased in SNL rats compared to na''ve and sham rats. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA, n = 7/group. (D - E): Western blot assay of VGLUT1 and VGLUT2 expression in CeA nucleus. Upper: representative of Western blot bands; Lower: analysis of the relative intensity of VGLUT1 and VGLUT2. β-actin is used as an internal control. Note that expression of VGLUT1 but not VGLUT2 is increased significantly in SNL rats compared to na''ve and sham rats. *p < 0.05, one-way ANOVA, n = 4 - 5/group.

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Molecular Brain

ISSN: 1756-6606