Figure 5

PKA phosphorylation of GluA1 in the ACC was important for the nerve injury induced mechanical hyperalgesia. (A) Diagram results showed the location of NASPM (filled circle) or saline (open circle) injection sites in the ACC. Bar equals to 200'µm. (B) Nerve injury induced mechanical hypersensitivity in wild type mice, which can be blocked by injection of NASPM into the ACC but not VS. (C) NASPM injection into the ACC or VS cannot change the mechanical threshold of mice with sham surgery. (D) NASPM's effect lasts for at least 4'hrs and disappeared 12'hrs after injection into the ACC in mice with nerve injury. (E) Summarized behavior results showing that nerve injury induced mechanical hypersensitivity is absent in s845A but not in s831A mice. (F) Nerve injury induced mechanical hyperalgesia was inhibited by PKA inhibitor Rp-cAMP consecutive injection into the ACC (before nerve ligation surgery, day 1, day 3, day 5 and day 7 after ligation surgery). ^#p >0.05, **p <0.01; ***p <0.001.