The suppression of AC5 in the dorsal striatum replicated behavioral preferences for KO food pellets and rough pellets. (A, B) Photomicrographs showing the dorsal striatum injected with siGLO control and high magnification of the rectangle (A). Real-time PCR showing siRNA-mediated knockdown of AC5 in the dorsal striatum (B). (C-F) The siRNA-mediated knockdown of AC5 in the dorsal striatum in WT mice induced behavioral preferences for KO pellets over WT pellets (C, D) and rough pellets over smooth pellets (E, F); the siRNA effects in both cases disappeared on day 4. Two-way repeated measures ANOVA, Holm-Sidak test: for Con-siRNA (C), time [F(2, 44) = 13.25, p <0.001], no food effect, and no interaction; for AC5-siRNA (D), no time effect, food [F(1, 18) = 15.73, p <0.001], and no interaction; for Con-siRNA (E), no time effect, no food effect, and significant interaction [F(2, 28) = 8.507, p = 0.0013]; for AC5-siRNA (F), time [F(2, 20) = 3.635, p = 0.0450], food [F(1, 10) = 19.89, p = 0.0012], and no interaction. (G, H) Lenti-AC5-shRNA-mediated knockdown of AC5 in the dorsal striatum in heterozygote AC5 KO mice (AC5+/-) mice induced preferred choices for AC5 KO-pellets over WT-pellets (G) and rough pellets over smooth pellets (H). Food-choice tests were started from 10 days after the Lenti-injection. Two-way ANOVA and Tukey s HSD test: for Lenti-AC5-shRNA effects on KO pellets (G), food [F(1,60) = 7.916, p = 0.007], no AC5-shRNA effect, and significant interaction [F(1,60) = 4.625 and p = 0.036]; for Lenti-AC5-shRNA effects on rough pellets (H), food [F(1,46) = 6.209, p = 0.0164], no AC5-shRNA effect, and no interaction. Mouse symbols: WT mice (black) with stereotaxic injections (arrows). Data were mean ± SEM (n = 11-19). * and ** denote difference between indicated groups at p <0.05 and p <0.01.