TWIK-1 contributes to outwardly rectifying currents in dentate granule cells. (A) Averaged current-voltage (I-V) relationships of whole-cell currents in dentate granule cells measured in standard ACSF and after subsequent application of Cs+/TEA. (B) Current-voltage relationship of the whole-cell currents in naïve (left panel; n = 34 cells, N = 3 mice), Sc shRNA (middle panel; n = 26 cells, N = 3 mice) or TWIK-1 shRNA (right panel; n = 31 cells, N = 3 mice) expressing dentate granule cells in the presence of Cs+/TEA. Whole-cell currents were elicited by 1 s duration ramp pulses descending from 40 mV to -150 mV from a holding potential of -70 mV. (C) Summary bar charts for (B). Shown are mean values of whole-cell current density in naïve, Sc shRNA or TWIK-1 shRNA expressing granule cells measured after subsequent application of the blockers; current density values are depicted at -150 mV (shadowed bars below baseline) and 40 mV (open bars). (D) Reversal potential values of the whole-cell currents in naïve (n = 34 cells, N = 3 mice), Sc shRNA (n = 26 cells, N = 3 mice) or TWIK-1 shRNA (n = 31 cells, N = 3 mice) expressing dentate granule cells. All data are represented as mean ± S.E.M; *P < 0.05, Student's unpaired t-test.