Impaired basal synaptic transmission and LTD induction in CreER-p35 cKO mice. (A, B) Input-output relationship. The slope of field excitatory postsynaptic potentials (fEPSPs) was significantly reduced in CreER-p35 cKO mice (control: n =17 slices [N =9 mice], p35 cKO: n =10 [N =5]) (A) with no change in presynaptic fiber volley amplitude (PSFV) (B) when compared with controls. *p<0.05, unpaired t-test. (C) Synaptic facilitation in response to paired-pulses with an inter-stimulus interval (ISI) of 25400ms (control: n =17 [N =9], p35 cKO: n =10 [N =5]). (D) Long-term depression (LTD) induced by low-frequency stimulation (LFS, 1Hz) combined with the application of the glutamate transporter blocker, tPDC. LTD was significantly impaired in CreER-p35 cKO mice when compared with controls. Insets are representative traces before and after LTD induction (control: n =7 [N =4], p35 cKO: n =8 [N =4]). (E) Long-term potentiation (LTP) induced by 3 theta-bust stimulation (3 TBS). LTP was comparable between genotypes. Insets are representative traces before and after LTP induction (control: n =17 [N =9], p35 cKO: n =10 [N =5]). Data represent mean ± S.E.M.