Figure 6
From: Cdk5/p35 functions as a crucial regulator of spatial learning and memory
Phosphorylation levels of GluR1 in CreER-p35 cKO mice. Western blots of (A) p35, pGluR1 (S845), GluR1 and NR2B, (B) pGluR1 (S831) and GluR1 (C) pCaMKII (T286), pCaMKII (T305) and CaMKII, (D) pCREB (S133) and CREB in the hippocampi of CreER-p35 cKO mice (n =4) and control (n =4). pGluR1 (S845, S831) are normalized to GluR1, pCREB (S133) normalized to CREB, and others normalized to actin. The phosphorylation level of pGluR1 (S845) does not change notably, but pGluR1 (S831) is significantly increased in CreER-p35 cKO (p35 cKO) when compared with p35-flox (control) mice. In CreER-p35 cKO mice, the phosphorylation level of pCaMKII (T286) is significantly increased in comparison with p35-flox mice. The phosphorylation of pCREB (S133) is not altered between the two genotypes. Error bars represent S.E.M. Unpaired t-test, *p<0.05, **p<0.01, ***p<0.001.