CR-positive neurons in the Sp5i and POm are not GABAergic interneurons. Double immunolabeling of anti-CR and GFP in GAD67-GFP mice at P8 indicates that GFP-positive neurons are located in the reticular thalamic nucleus (Rt) and are not co-labeled with CR (A). CR expression is specifically detected in the POm in the dorsal thalamus (B, C). In the medulla oblongata, CR-positive neurons and GFP-positive neurons can be detected in the Sp5i, but CR and GFP are not co-labeled (D-F). The insert represents an amplification of CR-positive neurons in the Sp5i. Scale bar: 100?m.