Statin treatment altered the secretory profile of microglia. Isolated rat microglial cultures were treated with 20 μM simvastatin for 24 h and subsequently activated with 1 μg/ml LPS overnight (18 h). For rescue experiments microglia were concurrently treated with 200 μM MEV for 24 h or subsequently treated with 1 mM hpβcd-cholesterol 30 minutes prior to LPS stimulus. Media was collected and assayed for cytokine release (Il1β and TNFα, A,D), neurotrophin release (BDNF, B), and nitric oxide secretion (C). All analyses were normalized to the total protein of the corresponding cell lysate to control for cell viability. Asterisks represent P <0.05, 0.01, and 0.001 by 2-way ANOVA and Bonferroni’s post-hoc from N = 6 independent preparations.