Table 1 Antibodies used for immunocytochemistry
Antigen | Host | Manufacturer | Dilution |
|---|---|---|---|
Ionized Ca 2+ -binding protein 1 (Iba1) | Rabbit | Wako | 1:1000 |
CD68 (ED-1 clone) | Mouse | Serotec | 1:750 |
Glial fibrillary acidic protein (GFAP) | Mouse | Sigma | 1:1000 |
GFAP | Rabbit | Dako | 1:1000 |
Mouse-Alexa Fluor 488 conjugate | Donkey | Life Technologies | 1:500 |
Rabbit-Alexa Fluor 488 conjugate | Donkey | Life Technologies | 1:500 |
Mouse-Alexa Fluor 647 conjugate | Donkey | Life Technologies | 1:500 |
Rabbit-Alexa Fluor 647 conjugate | Donkey | Life Technologies | 1:500 |