Myelin induces an IEG response of c-Fos involving SRF. Cerebellar neurons were stimulated with total myelin for the indicated time-points followed by analysis of c-Fos protein abundance. (A) Myelin induces c-Fos after 1 and 2 h of application. At later time-points, c-Fos is down-regulated as also depicted in the quantification of an individual experiment. (B) In Srf deficient neurons, myelin fails to induce c-Fos to a similar extent as observed in wild-type neurons. (C) Myelin recruits MAP kinases and Rho-GTPase but not cAMP/PKA signaling to induce c-Fos. Numbers in bars indicate independent cell cultures analyzed.