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Figure 3 | Molecular Brain

Figure 3

From: Human post-mortem synapse proteome integrity screening for proteomic studies of postsynaptic complexes

Figure 3

Affinity purification of the MAGUK Associated Signalling Complexes (MASC) from brain cortex and observed MASC composition in NSB or PM frontal cortex. A. Coomassie stained SDS-PAGE electrophoresis of MASC complexes isolated by affinity purification from mouse cortex, human NSB and PM frontal cortex. Human sample details in Additional file 1. MW: Molecular Weight markers; pep6, eluate from affinity purification using pep6 peptide; pep6δV, eluate from affinity purification using control peptide. B. Immunoblots of three members of the DLG4/PSD-95 family (DLG4/PSD-95, DLG3/SAP102 and DLG2/PSD-93) for 6 human MASC purifications (pep6), and corresponding controls (pep6δV); three from NSB and three from PM frontal cortex. C. Panther Protein Classes in the postsynaptic proteome (in green), the NSB MASC (in red) or the PM MASC (in blue) that are significantly enriched as compared with the human genome. Bar height corresponds with percentage of all proteins found in that Protein Class. (See Additional file 2 for methods and detailed bioinformatics functional analysis).

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