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Figure 2 | Molecular Brain

Figure 2

From: Genome-wide screen for modifiers of Na+/K+ATPase alleles identifies critical genetic loci

Figure 2

Schematic of the deficiency screen workflow. Using the Bloomington deficiency kit, 1137 initial interactions were screened using ATPalphaCJ5, ATPalphaCJ10, or ATPalphaDTS1. Putative enhancers and suppressors were selected for verification with a larger sample size. Any verified interacting deficiencies were deemed critical intervals. Once critical intervals were selected a screen for single gene modifiers from within the intervals was performed using available classical mutants and transgenic RNAi strains. If a modifier was found it was retested with other ATPalpha alleles to determine whether the interaction was allele-specific.

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