Figure 2From: Genome-wide screen for modifiers of Na+/K+ATPase alleles identifies critical genetic lociSchematic of the deficiency screen workflow. Using the Bloomington deficiency kit, 1137 initial interactions were screened using ATPalphaCJ5, ATPalphaCJ10, or ATPalphaDTS1. Putative enhancers and suppressors were selected for verification with a larger sample size. Any verified interacting deficiencies were deemed critical intervals. Once critical intervals were selected a screen for single gene modifiers from within the intervals was performed using available classical mutants and transgenic RNAi strains. If a modifier was found it was retested with other ATPalpha alleles to determine whether the interaction was allele-specific.Back to article page