Schematic of the deficiency screen workflow. Using the Bloomington deficiency kit, 1137 initial interactions were screened using ATPalphaCJ5, ATPalphaCJ10, or ATPalphaDTS1. Putative enhancers and suppressors were selected for verification with a larger sample size. Any verified interacting deficiencies were deemed critical intervals. Once critical intervals were selected a screen for single gene modifiers from within the intervals was performed using available classical mutants and transgenic RNAi strains. If a modifier was found it was retested with other ATPalpha alleles to determine whether the interaction was allele-specific.