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Table 5 Single gene effects confirmed for ATPalpha alleles using classical mutants

From: Genome-wide screen for modifiers of Na+/K+ATPase alleles identifies critical genetic loci

Cytological region

Gene

Genotype

Putative function#

ATPα Allele

Nature of interaction

Significance

10B3

l(1)10Bb

E04588

Spliceosome component [44]

CJ10

Suppressor

*

21B1-21B1

Galectin

DG25505

Cell surface protein, galactoside binding [45]

DTS1

Enhancer

***

23C9-23C9

Rbp9

∆1

RNA binding [46]

DTS1

Enhancer

*

23C9-23C9

Rbp9

∆1

"

CJ5

Suppressor

****

27E-28B1

Ndae1

MB05294

Sodium driven anion exchanger [47]

CJ5

Suppressor

*

27E-28B1

Ndae1

MB05294

"

DTS1

Enhancer

*

27E-28B1

Ndae1

MB05294

"

CJ10

Suppressor

*

29B4-29E4

Sema-1a

K13702

Axon guidance signal and receptor [48],[49]

DTS1

Enhancer

*

29B4-29E4

Glt

EY22126

Cell surface glycoprotein [50]

DTS1

Enhancer

****

29B4-29E4

Glt

EY22126

“

CJ10

Suppressor

*

30C7-30 F2

Nckx30C

E00401

Sodium/Calcium/Potassium exchanger [51]

CJ10

Enhancer

*

30C7-30 F2

Ppk11

MB02012

Excitatory sodium channel [52]

CJ10

Suppressor

***

30C7-30 F2

Ppk11

MB02012

“

DTS1

Suppressor

****

30C7-30 F2

Ppk11

MB02012

“

CJ5

Suppressor

****

30C7-30 F2

nAChRα6

MB06675

ACh receptor subunit

CJ10

Suppressor

*

30C7-30 F2

nAChRα6

MB06675

“

CJ5

Suppressor

****

31C-32E

FKBP59

EY03538

Calcium channel regulator [53]

DTS1

Enhancer

*

31C-32E

FKBP59

EY03538

"

CJ5

Suppressor

***

31C-32E

FKBP59

EY03538

“

CJ10

Suppressor

***

33A8-33B1

Pde1c

C04487

cAMP/cGMP phosphodiesterase [54]

CJ5

Suppressor

****

34E4-35B4

Dyrk2

1

Serine/Threonine kinase [55]

DTS1

Enhancer

***

34E4-35B4

Dyrk2

1

"

CJ10

Suppressor

****

34E4-35B4

Nimb5

MI01793

Bacterial defense

CJ10

Suppressor

**

34E4-35B4

nAChRα5

MB11647

ACh Receptor subunit

CJ10

Suppressor

*

35 F1-36A1

Cact

7

Inhibitor of NF-κB [56]

CJ10

Suppressor

****

36A8-36 F1

Beat-Ia & Fas3

3/E25

Neuronal immunoglobulin-like proteins

CJ5

Suppressor

*

25 F1-36A1

CG5888

MB00188

Toll 3 like receptor

DTS1

Enhancer

****

25 F1-36A1

CG5888

MB00188

"

CJ5

Suppressor

****

46 F1-47A9

CG42732

MB04544

Predicted potassium channel

DTS1

Enhancer

****

46 F1-47A9

Rpl41/NaCP60E

EP348

Ribosomal protein; voltage-gated Na+ channel [57]

CJ10

Suppressor

*

46 F1-47A9

CG42732

MB04544

Predicted potassium channel

CJ5

Suppressor

**

46 F1-47A9

Gαo

MI00833

Heterotrimeric G-protein subunit

CJ10

Suppressor

****

46 F1-47A9

CYP49A1 & Gαo

MB04922

Cytochrome P450 & heterotrimeric G-protein subunit

DTS1

Enhancer

****

50B1

CG33156

MB05931

Predicted NAD+ kinase

DTS1

Enhancer

****

57C5-57 F6

Pu

r1

GTP cyclohydrolase [58]

CJ5

Suppressor

**

57C5-57 F6

Pu

r1

“

CJ10

Suppressor

****

60E6-60E11

Pain

EP2451

TRP calcium channel [59]

DTS1

Enhancer

**

60E6-60E11

Pain

EP2451

“

CJ10

Suppressor

****

60E6-60E11

Rpl19

K03704

Ribosomal component [60]

DTS1

Enhancer

****

62E8-63B6

Spz5

E03444

Neurotrophin [61],[62]

DTS1

Enhancer

**

62E8-63B6

Spz5

E03444

“

CJ10

Suppressor

****

62E8-63B6

Aly

1

Regulator of transcription [63],[64]

DTS1

Enhancer

****

62E8-63B6

Rasp

m47

Palmitoyl transferase [65],[66]

DTS1

Enhancer

**

62E8-63B6

Rasp

m47

“

CJ10

Suppressor

****

63A3-63A3

Scramb2

EY01180

Predicted phosphatidyl serine scramblase

DTS1

Enhancer

****

63A3-63A3

Scramb2

EY01180

"

CJ10

Suppressor

****

67A2-67D13

Aay

S042314

Predicted Phosphoserine phosphatase

CJ10

Suppressor

**

93B9-93D4

Slmb

295

Ubiquitin ligase [67],[68]

DTS1

Enhancer

**

93B9-93D4

Slmb

295

“

CJ10

Suppressor

****

93B9-93D4

Sec15

2

Protein trafficking [69],[70]

DTS1

Enhancer

**

93B9-93D4

Sec15

2

“

CJ10

Suppressor

****

93B9-93D4

RhoGAP93B

EY07136

Rac1 GAP [71]

DTS1

Enhancer

*

98 F10-99B9

CG14508

G9163

Predicted cytochrome C

DTS1

Enhancer

***

98 F10-99B9

CG14508

G9163

Predicted cytochrome C

CJ10

Suppressor

****

99E1-3Rt

Sro

1

Ecdysone biosynthetic pathway

CJ10

Suppressor

*

  1. Many genes had an interaction with more than one allele, although some appear to be allele specific. Double mutants were compared to ATPalpha*/+ and heterozygous classical mutant controls. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
  2. #Function per flybase.org and / or listed citation.