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Table 5 Single gene effects confirmed for ATPalpha alleles using classical mutants

From: Genome-wide screen for modifiers of Na+/K+ATPase alleles identifies critical genetic loci

Cytological region Gene Genotype Putative function# ATPα Allele Nature of interaction Significance
10B3 l(1)10Bb E04588 Spliceosome component [44] CJ10 Suppressor *
21B1-21B1 Galectin DG25505 Cell surface protein, galactoside binding [45] DTS1 Enhancer ***
23C9-23C9 Rbp9 ∆1 RNA binding [46] DTS1 Enhancer *
23C9-23C9 Rbp9 ∆1 " CJ5 Suppressor ****
27E-28B1 Ndae1 MB05294 Sodium driven anion exchanger [47] CJ5 Suppressor *
27E-28B1 Ndae1 MB05294 " DTS1 Enhancer *
27E-28B1 Ndae1 MB05294 " CJ10 Suppressor *
29B4-29E4 Sema-1a K13702 Axon guidance signal and receptor [48],[49] DTS1 Enhancer *
29B4-29E4 Glt EY22126 Cell surface glycoprotein [50] DTS1 Enhancer ****
29B4-29E4 Glt EY22126 CJ10 Suppressor *
30C7-30 F2 Nckx30C E00401 Sodium/Calcium/Potassium exchanger [51] CJ10 Enhancer *
30C7-30 F2 Ppk11 MB02012 Excitatory sodium channel [52] CJ10 Suppressor ***
30C7-30 F2 Ppk11 MB02012 DTS1 Suppressor ****
30C7-30 F2 Ppk11 MB02012 CJ5 Suppressor ****
30C7-30 F2 nAChRα6 MB06675 ACh receptor subunit CJ10 Suppressor *
30C7-30 F2 nAChRα6 MB06675 CJ5 Suppressor ****
31C-32E FKBP59 EY03538 Calcium channel regulator [53] DTS1 Enhancer *
31C-32E FKBP59 EY03538 " CJ5 Suppressor ***
31C-32E FKBP59 EY03538 CJ10 Suppressor ***
33A8-33B1 Pde1c C04487 cAMP/cGMP phosphodiesterase [54] CJ5 Suppressor ****
34E4-35B4 Dyrk2 1 Serine/Threonine kinase [55] DTS1 Enhancer ***
34E4-35B4 Dyrk2 1 " CJ10 Suppressor ****
34E4-35B4 Nimb5 MI01793 Bacterial defense CJ10 Suppressor **
34E4-35B4 nAChRα5 MB11647 ACh Receptor subunit CJ10 Suppressor *
35 F1-36A1 Cact 7 Inhibitor of NF-κB [56] CJ10 Suppressor ****
36A8-36 F1 Beat-Ia & Fas3 3/E25 Neuronal immunoglobulin-like proteins CJ5 Suppressor *
25 F1-36A1 CG5888 MB00188 Toll 3 like receptor DTS1 Enhancer ****
25 F1-36A1 CG5888 MB00188 " CJ5 Suppressor ****
46 F1-47A9 CG42732 MB04544 Predicted potassium channel DTS1 Enhancer ****
46 F1-47A9 Rpl41/NaCP60E EP348 Ribosomal protein; voltage-gated Na+ channel [57] CJ10 Suppressor *
46 F1-47A9 CG42732 MB04544 Predicted potassium channel CJ5 Suppressor **
46 F1-47A9 Gαo MI00833 Heterotrimeric G-protein subunit CJ10 Suppressor ****
46 F1-47A9 CYP49A1 & Gαo MB04922 Cytochrome P450 & heterotrimeric G-protein subunit DTS1 Enhancer ****
50B1 CG33156 MB05931 Predicted NAD+ kinase DTS1 Enhancer ****
57C5-57 F6 Pu r1 GTP cyclohydrolase [58] CJ5 Suppressor **
57C5-57 F6 Pu r1 CJ10 Suppressor ****
60E6-60E11 Pain EP2451 TRP calcium channel [59] DTS1 Enhancer **
60E6-60E11 Pain EP2451 CJ10 Suppressor ****
60E6-60E11 Rpl19 K03704 Ribosomal component [60] DTS1 Enhancer ****
62E8-63B6 Spz5 E03444 Neurotrophin [61],[62] DTS1 Enhancer **
62E8-63B6 Spz5 E03444 CJ10 Suppressor ****
62E8-63B6 Aly 1 Regulator of transcription [63],[64] DTS1 Enhancer ****
62E8-63B6 Rasp m47 Palmitoyl transferase [65],[66] DTS1 Enhancer **
62E8-63B6 Rasp m47 CJ10 Suppressor ****
63A3-63A3 Scramb2 EY01180 Predicted phosphatidyl serine scramblase DTS1 Enhancer ****
63A3-63A3 Scramb2 EY01180 " CJ10 Suppressor ****
67A2-67D13 Aay S042314 Predicted Phosphoserine phosphatase CJ10 Suppressor **
93B9-93D4 Slmb 295 Ubiquitin ligase [67],[68] DTS1 Enhancer **
93B9-93D4 Slmb 295 CJ10 Suppressor ****
93B9-93D4 Sec15 2 Protein trafficking [69],[70] DTS1 Enhancer **
93B9-93D4 Sec15 2 CJ10 Suppressor ****
93B9-93D4 RhoGAP93B EY07136 Rac1 GAP [71] DTS1 Enhancer *
98 F10-99B9 CG14508 G9163 Predicted cytochrome C DTS1 Enhancer ***
98 F10-99B9 CG14508 G9163 Predicted cytochrome C CJ10 Suppressor ****
99E1-3Rt Sro 1 Ecdysone biosynthetic pathway CJ10 Suppressor *
  1. Many genes had an interaction with more than one allele, although some appear to be allele specific. Double mutants were compared to ATPalpha*/+ and heterozygous classical mutant controls. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
  2. #Function per flybase.org and / or listed citation.