Figure 1From: Nlrx1 regulates neuronal cell deathNlrx1 expression in N2A cells. (A) Representative photograph of Immunoblotting of KI, KD, and Sc cells, molecular weight of endogenous Nlrx1 is 108 kDa. In KI cells Nlrx1-GFP is around 130 kDa. (B) Nlrx1 protein expression in KI, KD, and Sc cells, n = 3. (C) Nlrx1 mRNA expression in KI (Nlrx1GFP), KD (shNlrx1), and Sc (Sh Scramble), n = 5. (D) Localization of Nlrx1 protein to mitochondria showing that Nlrx1 co-localized with mitochondria, but not with lysosomes. Photomicrographs of confocal images of cells labeled green for Nlrx1 and stained with mitotracker or lysotracker (red). Overlay showing co-localisation of Nlrx1 and mitotracker or with lysotracker (orange). * p < 0.0001.Back to article page