Nlrx1 promotes mitochondrial fission. Photomicrographs showing mitochondrial content. (A) KD Nlrx1 cells have a decreased mitochondrial number. (B) KI Nlrx1 cells have more mitochondria and those mitochondria have swollen cristae. * represent mitochondria, scale bar is 500 nm. (C) There is a higher mitochondrial mass in KI Nlrx1 cells compared to KD Nlrx1 cells as showed by Mitotracker red staining in GFP positive cells, n = 3. (D) Representative plot of flow cytometry experiment with Mitotracker red staining showing a higher mitochondrial mass in KI Nlrx1 cells compared to KD Nlrx1 cells.