Figure 5
Nlrx1 associates with DRP1. (A) Immunoblotting of DRP1 and Phosphorylated DRP1 at serine 616 in Sc, KI, and KD N2A cells. (B) Quantification of phospho-DRP1 normalised to total DRP1. There is more phosphorylated DRP1 at Ser616 in KI Nlrx1 cells compared to KD shRNA and Sc cells. (C) Immunoprecipitation of Nlrx1 and DRP1, but not of Nlrx1 and HSP90 or COX IV. Co-immunoprecipitation with anti-tGFP antibody in lane 5 and 6; and with anti-DRP1 antibody lane 3 and 4; and anti-P-DRP1 lane 1 and 2. Lane 1, 3, and 5 contain supernatant; lane 2, 4 and 6 contain IP fractions, and lane 7 contains total protein lysate. The Western blot was probed with anti-DRP1 antibody. * Significantly different from Sc, p < 0.05.