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Figure 3 | Molecular Brain

Figure 3

From: Protein tyrosine phosphatase receptor type R is required for Purkinje cell responsiveness in cerebellar long-term depression

Figure 3

ERK and MEK phosphorylation profiles following LTD induction in wild type and Ptprr −/− cerebellar slices. (A) Immunoblot detecting phospho-ERK1/2 (upper panel) and total ERK1/2 (lower panel) in cerebellar slices of wild-type and Ptprr −/− mice under basal conditions, after a 5-min stimulation with K-Glu to induce LTD, and following subsequent 10 or 20 min washing out of K-Glu. Representative images are shown. (B) Quantitative representation of phospho-ERK1/2 over total ERK1/2 levels (arbitrary units) in lysates of cerebellar slices as determined under (A). Results are presented as mean values and error bars indicate SEM from five independent experiments (Student’s t-test, *p < 0.05). (C) An alternative quantitative representation of the data determined under (A) showing the fold increase of phospho-ERK1/2-total ERK1/2 ratios in the different samples as compared to the ratio under basal conditions. Results are presented as mean values and error bars indicate SEM from five independent experiments (Student’s t-test, *p < 0.05). (D) Immunoblot detecting phospho-MEK1/2 (upper panel) and total MEK1/2 (lower panel) in cerebellar slices of wild-type and Ptprr −/− mice under basal conditions, stimulated with K-Glu to induce LTD, or after subsequent wash out of K-Glu for 10 or 20 min. Representative images out of five independent experiments are shown. (E) Quantification of the ratio of phospho-MEK1/2 over total MEK1/2 levels (arbitrary units) from the cerebellar slice lysates as described under (D). Results are presented as mean values and error bars indicate SEM from five independent experiments. (F) Quantitative representation of the fold increase of relative phospho-MEK1/2 levels compared to the relative levels under basal conditions as determined in the cerebellar slice lysates described under (D). Results are presented as mean values and error bars indicate SEM from five independent experiments (Student’s t-test, **p < 0.01).

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